Fig. 1. Localization of BAF in Drosophila cells and tissues. Localization of Drosophila BAF during the cell cycle was determined by double immunostaining with rabbit polyclonal anti-BAF antibodies (green, BAF) and either mouse monoclonal anti-lamin Dm₀ antibodies (red, LamDm0) (A,C,E) or rat monoclonal anti-phosphorylated histone H3 Ser²⁸ monoclonal antibodies (HTA28; red, P-H3) (B,D) in tissues from third instar larvae. Both low magnification (A,B) and enlarged images are shown (C,D,E). Colocalization is yellow (Merge). In A, anti-BAF antibodies were also tested for specificity by pre-incubation of antiserum with either thioredoxin-his-tag protein alone (TrH tag) or thioredoxin-his-tag-Drosophila BAF fusion protein (TrH-BAF) as indicated._. White arrows in B and D indicate condensed chromosomes. Scale bars: 20 µm (A,B) and 5 µm (C-E) All images were recorded with a confocal microscope.