Fig. 6. Nuclear lamin distribution in cells of larvae lacking the baf gene. The localization of nuclear lamin Dm₀-derivatives was evaluated by indirect immunofluorescence microscopy using a highly specific mAb. (A) Immunofluorescence images of the whole CNS from third instar larvae of baf¹/+, baf¹/baf¹ and baf¹/baf¹ animals rescued with gBAF (gBAF rescue). (B,C) Confocal immunofluorescence images of the third instar larval brain hemispheres of baf¹/+, baf¹/baf¹ rescued with gBAF, and baf¹/baf¹ animals. Obvious nuclear rim staining is found exclusively in the CNS of baf¹/+ and baf¹/baf¹ animals rescued with gBAF (B). In the case of baf¹/baf¹ CNS (C), abnormal lamin staining is observed in the left two panels. The convoluted nuclear lamin staining is apparent in brain hemisphere cells of baf¹/baf¹ animals at higher magnification (rightmost upper and lower panels in C). Br, brain hemisphere; Im, imaginal discs; Th, thoracic neuroblasts. Scale bars: 50 µm (A; all upper panels in and all lower panels are shown at the same magnifications, respectively) 20 µm (B: as in A); 20 µm (C, left) 5 µm (C, right).