Fig. 7. Comparison of BAF and lamin distribution in cells of larvae lacking the baf gene. The correlation between abnormal lamin distribution and BAF level was investigated by confocal immunofluorescence. (A,B) Third instar larval brain hemispheres and thoracic ganglia of baf¹/baf¹animals were double immunostained with rabbit polyclonal anti-BAF antibodies (green, BAF) and mouse monoclonal anti-lamin Dm₀ antibodies (red, LamDm₀). B is a higher magnification from a similar experiment to A. Antigen colocalization is yellow (Merge). White arrows in A indicate convoluted nuclei in which BAF cannot be detected with anti-BAF antibodies. Scale bar: 20 µm (A); 5 µm (B).