Fig. 5. Immunogold labelling of maturing schizonts with antibody against PfAMA-1 whole ectodomain. (A) An obliquely sectioned merozoite apex with a cluster of micronemes strongly labelled by Pab 1, surrounding the tips of two unlabelled rhoptries (rh). Scale bar, 100 nm. (B) Two micronemes (black arrows) labelled by Pab 3 and a longitudinally sectioned rhoptry devoid of labelling, including its narrow apical end (white arrow). Scale bar, 100 nm. (C,D) PfAMA-1 labelling by Pab 3 around the peripheries of micronemes. The microneme in (D) was situated near the Golgi cisterna and represents an early stage in this organelle's migration to the apex. Scale bars, 50 nm.