Fig. 6. Western blots of the polyclonal antibodies (Pab 1, Pab 2 and Pab 3, and control Pab 4) used to study PfAMA-1 expression in extracts of late-stage ITO4 schizont-parasitized red blood cells (p) and uninfected red blood cell (r) samples for each. Lanes: (A) p and r incubated with (control) antibody against Pichia expression contaminants; (B-D) incubated with anti-PfAMA-1 Pab 1 (B), Pab 2 (C) and Pab 3 (D). All anti-PfAMA-1 antibodies stain both the unprocessed (PfAMA-1₈₃) and processed (PfAMA-1₆₆) forms of PfAMA-1. Lower molecular weight bands represent further cleavage products, variably detected by the three anti-PfAMA-1 antibodies.