Fig. 1. LPA-stimulated cell migration. For wound healing experiments, cells were incubated until a confluent monolayer was formed. Cells were then serum-starved overnight in DMEM containing 0.1% BSA, treated for 2 hours with 10 µg/ml mitomycin C to inhibit cell proliferation, and a cell-free space was created by scraping through the monolayer. Migration was induced by addition of 10 µM LPA. Results from one representative experiment of three independent studies are shown.