Fig. 3. Ovulation defects in talin RNAi hermaphrodites. Nematodes were grown on HT115(DE3) with empty vector (A,C) or with pPD129.36-talin (B,D) for 52 hours. (A) DIC image shows normal linear arrangement of oocytes in the proximal gonad arm. (B) This arrangement contrasts with stacking of oocytes in a distended gonad arm of talin RNAi animals. (C) Normal DNA organization in the germ cells and oocytes (arrows) was visualized by DAPI staining. (D) Talin RNAi animals had stacked oocytes in the proximal gonad arm (arrows) and germ line nuclei were observed throughout the gonad arm. Brackets in C and D indicate location of sperm. All animals are anterior to the left, ventral down. Scale bars: 25 µm.