Fig. 4. Actin filament organization is disrupted in the gonad sheath cells. Rhodamine-conjugated phalloidin was used to stain actin filaments in dissected gonad arms of (A) animals grown on bacteria carrying the empty RNAi vector or (B) talin RNAi animals for 48 hours. (A) Proximal gonad arm has longitudinal actin filaments in the sheath cells. This pattern is absent from the proximal arm of a talin RNAi gonad (B, left), but actin filaments appear normal in distal gonad arm (B, right). Scale bars: 25 µm.