Fig. 6. PAR-3 localizes at cell-cell contacts of endothelial cells. (A) Human umbilical vein endothelial cells (HUVEC) were stained with antibodies against PAR-3, ZO-1 and AF-6. Bound antibodies were visualized with biotinylated donkey anti-rabbit IgG and Cy3-conjugated streptavidin. PAR-3 localizes at cell-cell contacts of HUVECs in a similar way to ZO-1 and AF-6. Bar, 20 µm. (B) Double-label immunofluorescence staining of HUVEC with antibodies against PAR-3 and JAM-2. Rat anti-JAM-2 antibodies were visualized with goat anti-rat FITC before further processing for incubations with rabbit anti-PAR-3 and goat anti-rabbit Texas Red in the presence of 0.2% of normal rat serum. PAR-3 colocalizes with JAM-2 at cell contacts of HUVECs. Bar, 25 µm.