Fig. 2. Internalisation of IgG-bound MT1-MMP complexes in bbHT1080 cells visualised by immunofluorescence microscopy. Intact and nonstimulated bbHT1080 cells were incubated with affinity-purified anti-MT1-MMP IgGs for 1 hour at 4°C. Cells were then washed and fixed immediately with paraformaldehyde (A) or warmed to 37°C for 10 (B), 20 (C), 30 (D) or 60 minutes (E) before fixation. In F, bbHT1080 cells were warmed to 37°C for 60 minutes in the presence of nocodazole to depolymerise microtubules. IgG-bound MT1-MMP complexes were detected using fluorescently labelled secondary antibody in TX-100-permeabilised cells. Bar, 20 µm.