Fig. 3. Internalised MT1-MMP colocalises with different endocytic markers. bbHT1080 cells that had internalised IgG-bound MT1-MMP complexes for 30 (A-F), 45 (G-I) or 60 minutes (J-L) were fixed, permeabilised and incubated with antibodies directed against EEA1 (B), Eps15 (E), Rab4 (H) and LAMP-1 (K). Arrowheads are examples of colocalising structures. The merged panels (C, F, I and L) depict colocalisation between MT1-MMP and the appropriate endocytic marker. Bar, 20 µm.