Fig. 5. Active MT1-MMP is internalised in EGFP-Eps^E95/295-expressing cells. bbHT1080 cells transiently transfected with a dyn^K44A dominant-negative dynamin mutant (A-D) or with an EGFPEps^E95/295 dominant-negative Eps15 mutant (E-H) were allowed to internalise anti-MT1-MMP pAb (A,B,E,F) or Texas red-conjugated transferrin (C,D,G,H) at 37°C. After fixation and TX-100 permeabilisation, IgG-bound MT1-MMP complexes (B,F) were revealed using a Texas-red conjugated secondary antibody. Bar, 20 µm.