Fig. 5. Subcellular localization of the Hrp65-2 isoform in C. tentans cells. (A) Immunoreactivity of the anti-Hrp65-2 antibody analyzed by western blot analysis of nuclear and cytoplasmic protein extracts prepared from C. tentans tissue culture cells. The proteins in each extract were separated by SDS-PAGE and blotted to transfer membranes. The membranes were cut into strips and incubated with the anti-Hrp65-2 antibody. The mobilities of molecular mass standards, in kDa, are shown on the left. (B) Semi-thin cryosections of C. tentans salivary gland cells were stained with the anti-Hrp65-2 specific antibody followed by a FITC-conjugated secondary antibody. An illustration of the subcellular structures that are visible after immunofluorescent labeling is shown in the middle. Cp, cytoplasm; chrom, polytene chromosomes; n, nucleolus; np, nucleoplasm. The broken line represents the nuclear envelope and the solid line represents the cell surface. The pre-immune serum was used in parallel as a negative control, and the picture was overlaid with a broken line to demarcate the border between nucleus and cytoplasm.