Fig. 4. Cell-cycle-dependent binding of HsMcm2p-HsMcm7p at oriP. Different cell cycle phases were separated by centrifugal elutriation and five cell cycle fractions were subjected to ChIP (G1, 40 ml minute^-1; G1/S, 50 ml minute^-1; S, 60 ml minute^-1; S/G2, 80 ml minute^-1; G2/M, 90 ml minute^-1). Cross-linked chromatin of 1x10⁷ cells was used for each immunoprecipitation. Co-precipitated DNA was isolated and 1/50 thereof was used for each PCR. Antibodies directed against HsMcm3p (A) and HsMcm7p (B) were used to visualize the cell-cycle-dependent binding of this complex. The values are calculated from three independent experiments.