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Fig. 1. Adult ventricular cardiomyocytes in culture. (A) Micrograph of an isolated cardiomyocyte in suspension after dissociation. Note the branched morphology and striated myofibrils. (B,C) Isolated cardiomyocytes after plating. Note the cross striations and expression of the myofibril markers MyHC (green, B and C) and troponin (red, B) or titin (red, C), as visualised by double label indirect immunofluorescence. (D) An interconnected group of cells, at 8 days after plating onto laminin, that beat in synchrony. (E,F) More than 90% of the cells in the culture are cardiomyocytes; expression of the myofibril markers troponin (E) and MyHC (F) at 8 days after plating (DNA stained with Hoechst 33258 in blue). Scale bar: 50 µm.