Fig. 2. Recruitment of parkin to the centrosome was dependent on the duration and dosage of lactacystin treatment. Representative confocal images from SHSY5Y cells treated with 10 µM lactacystin for 0, 4, 8 and 12 hours (A-D). Time-dependent increase of parkin (green) was seen in the centrosome, where -tubulin (red) signals overlapped (yellow) with some of parkin signal. The area of the -tubulin staining also increased with time of treatment. Scale bars: 5 µm. (E) The average area of parkin accumulation was plotted against the duration of lactacystin (10 µM) treatment. #P<0.05 vs. 0 hour; ^*P<0.001 vs. 0 hour (n=19 cells for each point). (F) The average area of parkin accumulation was plotted against the concentration of lactacystin treated for 12 hours. ^*P<0.001 vs. 0 µM (n=16 cells for each point).