Fig. 3. Lactacystin did not cause a general recruitment of proteins to the centrosome. No significant centrosomal accumulation was seen for CREB (CREB; A,B) or MAP kinase (MAPK; C,D) in SH-SY5Y cells treated without (-Lac; A,C) or with (+Lac; B,D) 10 µM lactacystin for 12 hours. The experiments were repeated three times with similar results. Scale bars: 5 µm.