JCS -- Nakano et al. 116 (19): 4021 Figure IG1

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Fig. 1. Cell lines obtained by transfection of an alphoid YAC constructs into HT1080 cells. (A) Following transfection of ${alpha}$ 21-I alphoid YAC ( ${alpha}$ 7C5hTEL) DNA, the de novo assembly of functional centromeres was observed coincident with MAC formation. MAC formation was not observed for transfection with ${alpha}$ 21-II alphoid YAC ( ${alpha}$ B13hTEL). 7C5HT1-19 is a subclone of a MAC-containing cell line (7C5HT1). (B) The structure and copy number of the alphoid YAC in the 7C5HT1 and derivative cell lines were determined by Southern blot analysis (Ikeno et al., 1998; Masumoto et al., 1998).