Fig. 2. Interphase centromere DNA clustering is impaired in mis6 cells and declustered centromeres contain the heterochromatin marker Swi6 but lack the kinetochore component Ndc80. (A) Images showing cen (otr)-FISH and Ndc80 localization in wild-type and mis6 cells at the permissive and restrictive temperatures. Scale bar: 0.40 µm. (B) Diagram showing the percentage of declustered centromeres. Symbols below the diagram show that the four categories of bars represent cells with normal centromere clusters, cells with one declustered centromere, cells with two declustered centromere FISH signals and finally cells with three declustered centromere FISH signals. (C) Images showing the location of the chromodomain protein GFP-Swi6 in wild-type cells and mis6 cells, respectively. (D) The area of cen(otr)-FISH signals represented as a bar diagram. The area in rik1-302 at 25°C (strain Hu607) was 0.8±0.4 µm, mis6 (Hu633) at 36°C was 0.4±0.2 µm, mis6 at 25°C was 0.2±0.1 µm and wild type (Fy2214) at 25°C was 0.2±0.1 µm (n=50).