Fig. 3. EM analysis of centromeres in mis6 and rik1 cells. Cells were subjected to HPF EM and immuno-gold staining for GFP-Swi6. (A,D) Different magnifications of a wild-type cell labeled with GFP-Swi6 immuno-gold. The strain used was Fy2214. (B,E) Two different magnifications of a mis6 cell grown at 36°C prior to fixation and labeled with GFP-Swi6 immuno-gold. Declustering of centromeric heterochromatin is seen. The strain used was Hu633. (C,F) Two different EM magnifications of a rik1 cell where electron dense heterochromatin is not readily detectable. Immuno-gold staining of GFP-Swi6 was not performed on this sample. The strain used was Hu607. HET, heterochromatin; N, nucleolus; SPB, spindle pole body. Swi6-Au, 10 nm gold labeling shows the presence of GFP-Swi6. Scale bar: 490 nm.