Fig. 5. Analysis of cen1::lacO-LacI-GFP and SPBs during mitosis in live wild type, nuf2 and mis6 cells. Images from time lapse analysis of live cells showing the location of cen1::lacO-LacI-GFP (green) and the SPB marker Cut12-CFP (red) in (A) wild-type (B) mis6 and (C) nuf2-1 cells. Scale bar: 0.2 µm. The white and red arrows indicate the positions of the SPB Cut12-CFP signals (red arrows indicate inferred SPB positions from other images in the time course series) and the yellow arrows indicate the position of cen1::lacO-LacI-GFP in cases where labeling was weak. The cytoplasmic red signal is nonspecific and due to autofluorescence detected with the CFP filter. Statistical analysis of anaphase cen1::lacO-LacI-GFP movements in live wild-type, mis6-302 and nuf2-1 cells is shown in Table 2.