Fig. 5. Effect of a low TGF-ß1 concentration on 6 (CD49f) and ß1 (CD29) integrin expression by keratinocytes. Long-term cultures were initiated with Adh⁺⁺⁺EGF-R^-/+ cells and performed with or without 10 pg/ml TGF-ß1. Cells at passage 4 were plated on glass slides, grown for 72 hours, and then processed for immunofluorescence. Expression of integrins was analyzed by Laser Scanning Cytometry (LSC) and compared in the two culture conditions. (A) Isotypic control corresponding to 6 integrin labeling. (B) Isotypic control corresponding to ß1 integrin labeling. (C) 6 integrin expression profile of keratinocytes grown in the control condition. (D) ß1 integrin expression profile of keratinocytes grown in the control condition. (E) 6 integrin expression profile of keratinocytes grown in the presence of 10 pg/ml exogenous TGF-ß1. (F) ß1 integrin expression profile of keratinocytes grown in the presence of 10 pg/ml exogenous TGF-ß1. Representative labeling profiles from a typical experiment are presented (n=3).