Fig. 1. HGF/SF does not regulate nuclear expression of Sp1 and Sp3 transcription factor in HaCaT cells. Representative western blot analysis of nuclear Sp1 and Sp3 expression by quiescent HaCaT cells, either left untreated (media change) or stimulated by HGF/SF for the indicated time periods. Total cellular protein (10 µg/lane) was separated by 10% SDS-PAGE, Sp1 and Sp3 protein were detected by enhanced chemiluminescence using specific antibodies to the respective nuclear factors (Santa Cruz; molecular weights are indicated to the right). Comparable results were obtained from three independent experiments.