Fig. 1. Effects of various inhibitors for PI3K, MLCK and F-actin on binding and phagocytosis of IgG-Es. Macrophages were fed IgG-Es in RB medium containing 0.1% dimethyl sulfoxide (Control), 50 µM LY294002 (LY294), 100 nM wortmannin (Wort), 10 µM ML-7, 20 µM ML-7 or 10 µM cytochalasin D (CyD). After 30-minute incubation at 4°C (for binding; white bars) or 37°C (for phagocytosis; black bars), the binding/phagocytic index (IgG-Es per cell) was scored as described in Materials and Methods. Bars show the mean and standard deviation of triplicate determinations (n=3) in a single experiment. Data are representative of at least three independent experiments.