Fig. 8. Digital image analysis of ruffling activities in control (A), wortmannintreated (B), ML-7-treated (C) and cytochalasin-D-treated cells (D). Subtracted images (right) were obtained by subtracting the images (center) from the frames taken 20 seconds earlier (left), then adding 120 to the gray value of each pixel. In subtracted images, the standard deviations (SD) of gray values of the cell area are shown. Bar, 10 µm.