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Fig. 4. Sequence analysis of the 5'-end of thi1 mRNA. (A) Evidence for a stable stem-loop structure in a region between the two in-frame start codons (boxed). Predicted secondary structure of the 90 first codons of the wild-type thi1 mRNA. Structure was generated for 28°C by using the mfold version 2.3 program (http://mfold2.wustl.edu/~mfold/rna/for1-2.3.cgi). Black dots indicate G:C base pairs and grey dots indicate weaker A:U or G:U base pairing. (B) Complementary sequence between thi1 mRNA and 18S rRNA of A. thaliana. Vertical lines indicate base pairing and open circles represent G:U base pairing.