Fig. 5. Analysis of CD14 expression in BMGs from TLR2^+/+ and TLR2^-/- mice. BMGs from TLR2^+/+ and TLR2^-/- mice were incubated for 24 hours at 37°C with the B. pertussis LPS, or with LPSs from the different strains of L. pneumophila. (A) CD14 expression in BMGs incubated with 1 or 10 µg/ml of the LPSs was detected by FACS analysis of the gated granulocyte population, as described in the legend to Fig. 3. The dashed line represents the level of CD14 in non-stimulated cells. (B) CD14 expression in BMGs incubated with 10 µg/ml of re-purified LPSs (re-extracted with phenol/deoxycholate) was analyzed by western blot in cell lysates, as described in the legend to Fig. 1.