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Fig. 6. CD14 expression induced by lipid A fractions in BMGs from TLR4- and TLR2-deficient mice. BMGs from C3H/HeJ, TLR2+/+ and TLR2-/- mice were incubated for 24 hours at 37°C with lipid A from B. pertussis (10 µg/ml), or from the different strains of L. pneumophila (50 µg/ml). CD14 expression was analyzed by western blot in cell lysates, as described in the legend to Fig. 1.