Fig. 8. Inhibitory effect of B. pertussis lipid A in C3H/HeJ cells. BMGs from C3H/HeJ mice were preincubated (90 minutes, 37°C) in the presence of various concentrations of B. pertussis lipid A. (A) The cells were reincubated (20 hours, 37°C) with (O) or without (•) LPS (1 µg/ml) from L. pneumophila CS338, and CD14 was detected by FACS analysis of the gated granulocyte population, as described in the legend to Fig. 3. (B) The cells were reincubated (20 hours, 37°C) with or without the lipid A fraction of L. pneumophila (0.1 µg/ml), and CD14 expression was analysed by western blot as described in the legend to Fig. 1.