Fig. 1. Deletion of the coilin self-association domain affects epitope recognition. (A) Schematic of human coilin showing the location of the self-interaction/CB-targeting domain (Hebert and Matera, 2000), two nuclear localization signals (NLS), nucleolar localization signal (NoLS) (Hebert and Matera, 2000) and the RG box that mediates interaction with SMN (Hebert et al., 2001). The regions of coilin that were used to generate anti-coilin antibodies R288 (Andrade et al., 1993) and R508 (Chan et al., 1994) are also indicated. (B) Coilin lacking the self-association domain is not recognized by anti-coilin Ab R508. HeLa cells transfected with myc-tagged coilin lacking the first 93 amino acids were subject to staining with anti-myc (left panels) or anti-coilin (right panels) antibodies.