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Fig. 7. Endocytosis in K-562 cells. K-562 cells were co-incubated with BODIPY~RNase A (1 µM) and either FM1-43 (1 µg/ml) or BODIPY-FL-labeled transferrin (50 µg/ml) for 20 minutes at 4°C. Cells were pulsed at 22°C for 15 minutes, washed and fixed. The fluorescence of BODIPY~RNase A (red), FM 1-43 (green) and BODIPY-FL-labeled transferrin (green) was visualized directly.