Fig. 9. Dynamin-independent internalization of RNase A. (A) Induced and uninduced K44A Dyn cells were co-incubated with OGRNase A (1 µM) or TAMRAtransferrin (50 µg/ml) for 20 minutes at 4°C. After a 15 minute incubation at 22°C, cells were washed and fixed; and the fluorescence of OGRNase A (green) and TAMRAtransferrin (red) was visualized directly. (B) Fractions of total uninduced (white) or induced (hatched) cells that contained internalized OGRNase A or TAMRAtransferrin. Data are the mean (±s.e.) of two separate experiments with n=65 and 105 cells for uninduced and induced samples, respectively.