Fig. 1. Immunofluorescent labelling of the PcG proteins in cultured cells. Confocal optical section through the central part of the nucleus. (A-E) SW480 cells labelled with anti-BMI1 (A), anti-HPC2 (B) and DAPI (C); (D) shows the merged image and (E) a line scan along the white arrow in panel D, showing the distribution of BMI1 (red line), HPC2 (green line) and DAPI (blue line). (F-G) SW 480 cells labelled with anti-RING1 (F) and DAPI (G). (H-J) T24 cells labelled with anti-BMI1 (H) and DAPI (I); J shows a line scan along the white arrow in H, showing the distribution of BMI1 (green line) and DAPI (blue line). (K-M) T24 cells labelled with anti-HPH1 (K) and DAPI (L); (M) shows a line scan along the white arrow in K, showing the distribution of HPH1 (green line) and DAPI (blue line).