Fig. 2. RNAi-mediated depletion of DAXX results in increased levels of apoptosis. A percent apoptosis is calculated as the percentage of total cells comprising the sub-G1 peak as determined by FACS analysis cell-cycle profiling following propidium iodide staining. Averages and standard deviations were calculated from a minimum of two independent transfection experiments. (A) HeLa cells were fixed and stained 48, 72 or 96 hours post-RNAi treatment. (B) HeLa cells were fixed and stained 72 hours post-RNAi treatment. (C) Western blot analysis of PARP in HeLa cell extracts prepared 96 hours following RNAi treatment. Full-length PARP migrates at 112 kDa; cleaved PARP migrates at 85 kDa.