Fig. 3. Bcl-2 rescues apoptosis in cells depleted of DAXX by RNAi. Mock- or hDx2-transfected HeLa cells were fixed and stained 72 hours post-RNAi treatment. DNA transfections were performed 4 hours after RNAi treatment. Percent apoptosis is calculated as the percentage of total cells comprising the sub-G1 peak as determined by FACS analysis cell-cycle profiling following propidium iodide staining. Averages and standard deviations were calculated from a minimum of two independent transfection experiments. In B, the sub-G1 peak of GFP-positive cells only is calculated.