Fig. 4. Depletion of DAXX by RNAi results in transcriptional de-repression. HeLa cells were transfected with RNAi (or mock transfected), followed 24 hours later by DNA transfection with Met-Luciferase and ß-gal constructs. Luciferase activity was measured after an additional 24 hours. In A, fold-de-repression was calculated by the fold increase of luciferase activity for hDx2-transfected relative to mock-transfected samples, with the mock-treated luciferase being set arbitrarily as 1. In B, DNA transfections in some cases included mouse Daxx cDNA (mDaxx), with the indicated microgram amount per well of six-well plate. All values represent averages of three independent transfections with standard deviation. All experiments were normalized by co-transfection with ß-gal.