Fig. 5. Depletion of DAXX by RNAi induces target-specific transcriptional de-repression. (A) HeLa cells were transfected with hDx2 or mDx2 followed 24 hours later by DNA transfection with reporter plasmids. Fold de-repression was calculated by the fold increase of luciferase activity for hDx2-transfected relative to mDx2-transfected samples, with the mDx2-treated luciferase value for each reporter being set arbitrarily as 1. (B) HeLa cells were transfected with reporter plasmids, with or without mouse Daxx cDNA. Fold repression is calculated by computing the fold difference between cells transfected without DAXX (mock transfected) relative to cells transfected with Daxx. All values represent averages of three independent transfections with standard deviation. All experiments were normalized by co-transfection with ß-gal.