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Fig. 5. NE- and ionomycin (ION)-induced CaMKII{alpha} activation is attenuated by inhibitors of CaM and CaMKII, and not their structural analogues. VSMCs were treated with NE (10 µM) (A,C), ionomycin (1 µM) (B,D) or vehicle (VEH) in the presence of inhibitors of CaM (10 µM W-7) (A,B), CaMKII (10 µM KN-93) (C,D), their corresponding structural analogues (10 µM W-5, 10 µM KN-92) or their vehicles (V) as described in Materials and Methods. CaMKII{alpha} activity was determined by immunoblot analysis using a polyclonal antibody against active CaMKII{alpha} (phospho-CaMKII{alpha}). The figure shows a representative blot and the densitometric analysis of three experiments performed with each inhibitor and their vehicles on different batches of cells grown in 100 mm tissue culture dishes. The density of CaMKII phosphorylation was quantified using NIH Image 1.62 (n=3). *Value significantly different from the corresponding value obtained with V of NE (A,C) or ION (B,D) (P<0.05).