Fig. 5. Temporal patterns of Ca²⁺ changes induced by 5-HT and Ins(1,4,5)P₃. The oocyte injected with 10 kDa (A, into the cytoplasm) or 70 kDa CGD (B, into the nucleus) was exposed to 5-HT (final concentration: 100 nM) in FSW. In C, the oocyte injected with 10 kDa CGD plus 600 µM caged Ins(1,4,5)P₃ was irradiated with UV light six times (arrows) in CaFSW. The F/F₀ values were calculated in the whole oocyte (A,C) or in the nuclear region alone (B). An inset in A depicts the expanded Ca²⁺ pattern of the first transient just after the addition of 5-HT. GVBD did not occur in A or B. In contrast, GVBD occurred in C just after the recording period.