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Fig. 2. (A) Flow cytometric analysis of CHO-Ncad and CHO-A5 transfectants. Note the significant shift in mean channel fluorescence of positive cells, representing approximately a 100-fold increase in protein expression. (B) Western blot analysis of CHO-Ncad and CHO-A5 transfectants. 25 µg of protein from cell lysates of CHO-Ncad and CHO-A5 cells were separated by SDS-PAGE, blotted to PVDF and subjected to immunoblot analysis using appropriate antibodies. Enhanced chemiluminescence detected a weak 130 kDa band corresponding approximately to the known average molecular weight for N-cadherin in the CHO-B2 parent line. A much stronger 130 kDa band is evident in the CHO-Ncad transfectant. {alpha}5 integrin was undetectable in CHO-B2 cells but was strongly expressed by the CHO-A5 cell line as represented by a 120 kDa band.