Fig. 9. Dose-dependent aggregation of FN-null CHO-5 cells. CHO-5 cells express high levels of 5 integrin but do not express N-cadherin or secrete FN. Cells were cultured in hanging drops either in the absence of FN (A) or in 3 (B), 30 (C), 100 (D) or 300 (E) µg/ml rat plasma FN. Note the dose-dependent aggregation and compaction of CHO-5 cells in response to the addition of exogenous FN. Bar, 0.5 mm.