Fig. 1. Immunofluorescence analysis of hemidesmosomal components in PA-JEB/ß4 cells. PA-JEB/ß4 cells were fixed and immunolabeled for laminin-5 (A) or the hemidesmosomal markers ß4 (A,B,D,F), plectin (B,G,I), BP180 (C,E,F) or BP230 (C,H,I). Merged images (A,B,C,F,I) show laminin-5, plectin and BP180 in green, and ß4 and BP230 in red; co-localized staining appears in yellow. Note that ß4 and plectin (B), as well as BP230 and BP180 (C), are nearly completely co-localized, whereas ß4 and BP180 (F) or plectin and BP230 (I) show only partial co-localization. Bar, 10 µm.