Fig. 4. Effects of expression of BP180 mutants on the localization of BP230 in GABEB keratinocytes. GABEB keratinocytes were transfected with cDNA encoding BP180^145-230 (A,B) or BP180^1-36 (C,D) and immunolabeled for BP180 (A-D), ß4 (A,C) or BP230 (B,D). In the merged images (A,C), BP180 is in green and ß4 in red, whereas in B and D, BP180 is in red and BP230 is in green. Staining for co-localization is in yellow. Diagrams at the bottom of panels A-D represent the various BP180 mutants. Note that deletion of amino acids 145-230 from BP180 does not abrogate the ability of BP180 to be recruited into HDs, whereas the recruitment of BP230 is impaired. Deletion of the N-terminal 36 residues of BP180 has no effect on the recruitment of either BP180 or BP230 into HDs. Bar, 10 µm.