Fig. 6. Expression of BP230 mutants in PA-JEB/ß4 keratinocytes. PA-JEB/ß4 cells were transfected with cDNAs encoding BP230^1-887 (A,B), BP230^1-2161 (C,E) or BP230^1-2161,ZY (D,F), tagged at the N-terminus with an HA epitope (red circle). Cells were fixed and immunolabeled for BP180 (A-D), the HA-tagged BP230 mutants (A-F) and total BP230 (endogenous and transfected BP230) (E,F). In the merged images (A-D), BP180 is in green and the HA-tagged mutants are in red, whereas in (E,F), the HA-tagged BP230 mutant is in green and total BP230 is in red. Co-localized staining appeared in yellow. Diagrams at the bottom of panels A-F represent the various BP230 mutants. Note that the cell depicted in B represents a rare event, whereas the majority of the cells resemble those depicted in A. Also note that removal of the Z-Y domains results in the loss of BP230 recruitment into HD-like structures. Bars, 10 µm.