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Fig. 7. The effect of Sec31 antibodies on PCTV budding. The budding assay was performed in the absence (-) or presence (+) of native cytosol (1 mg protein) plus pre-immune IgG and cytosol (1 mg protein) treated with 3 µl of anti-Sec31 antibodies (Sec31). Data from fractions 1-20 are shown since fractions 21 to 70 had no 3H-dpm over baseline.