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Fig. 6. Effects of ADF/cofilin isoforms and mutants on UNC-78-dependent actin filament assembly. F-actin (10 µM) was incubated for 30 minutes with UNC-78 (0-6 µM) and UNC-60B (A), UNC-60A (B), mouse M-cofilin (C), UNC-60B (A111V) (D), UNC-60B (S112F) (E), or UNC-60B (Q150*) (F), and subjected to pelleting assays. Percentages of actin in the pellets were quantified and plotted as a function of the concentration of ADF/cofilin proteins. Data shown are mean±s.d. of three experiments.