Fig. 7. Specificity of anti-UNC-78 antibody and expression of UNC-78. Total worm lysates (25 µg proteins) of wild type (lane 1), unc-78 (gk27) (unc-78 null) (lane 2), and unc-60 (su158) (unc-60B null) (lane 3) and purified recombinant UNC-78 protein (0.1 µg) (lane 4 only in B) were resolved by SDS-PAGE (10% acrylamide gel) and visualized by Coomassie Blue (A) or subjected to western blot with anti-UNC-78 (B), anti-actin (C), or anti-UNC-60B (D). Molecular mass markers in kDa (lane M) are indicated on the left of A.