Fig. 1. Mitotic cyclin localization. (A) Localization of the Clb1, Clb2, Clb3 and Clb4 mitotic cyclins. The indicated mitotic cyclins were expressed as GFP fusions under the control of the GAL1 promoter. Cells grown in SC-raffinose were collected on nitrocellulose filters and induced for 3 hours on YEP-galactose plates before microscopic observation. (B,C) Clb2 localizes both on the spindle pole bodies and along the mitotic spindle. Cells expressing Spc29-CFP and Clb2-YFP or Tub1-CFP and Clb2-YFP are shown. CLB2-YFP was overexpressed from the GAL1p as described below. The left hand column is YFP, the center column is CFP and the right hand column shows merged YFP/CFP. (D) Localization of chimeras between Clb2 and Clb3. Cellular localization of the chimeras fused to GFP is shown. Galactose induction of the GFP-fused chimeras was achieved as described in A.