Fig. 6. Cytokinesis is delayed in bud3 and bud3¹²²¹ cells. (A) Cells (15DU, MNY252, MNY264) were arrested with 60 ng/ml -mating factor for 2 hours and released synchronously at 25°C. Aliquots were taken every 15 minutes and DNA content analyzed by flow cytometry. (B) The proportion of cells entering a second round of budding before cell separation (arrow) was scored at the indicated times. (C,D) Cells were synchronized as in A but a second cell cycle was prevented by adding back 60 ng/ml -factor at t₇₅ min. The percentage of binucleate cells (C) and budded cells (D) was determined microscopically after a brief sonication.