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Fig. 1. The domain structure of the RIN members and northern blot analysis of RIN3 mRNA in human tissues. (A) Diagram of the structural features of the RIN-family members. The numbers at the bottom represent the amino acid residues. (B, top) The probe corresponding to the 729-base RIN3 cDNA was labelled by random priming and hybridized to a human multiple tissue RNA blot containing 2 µg lane-1 of poly(A) mRNA from various human tissues. (B, bottom) The northern blot was also performed with actin cDNA as a control.